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7.10 Water: Leaves → atmosphere

Arrival of Water at the Leaves

  • Xylem Vessels in Leaves:
    • Location: Water arrives at the leaves through xylem vessels located within the vascular bundles (veins).
    • Connection: Xylem vessels in leaves are continuous with those in the stem, ensuring an uninterrupted pathway for water transport.

Exit of Water from Xylem into Leaf Tissues

Movement from Xylem to Bundle Sheath Cells

  1. Pits in Xylem Vessel Walls:
    • Description: Areas where lignin is absent, creating pores in the xylem vessel walls.
    • Function: Allow water to move out of the xylem into adjacent bundle sheath cells.
  2. Bundle Sheath Cells:
    • Role: Act as intermediary cells facilitating the transfer of water from xylem to mesophyll cells.

Pathways from Bundle Sheath to Mesophyll Cells

  • Symplast Pathway:
    • Description: Water moves from bundle sheath cells into mesophyll cells through plasmodesmata (cytoplasmic channels).
    • Significance: Allows regulated and selective movement of water and solutes.
  • Apoplast Pathway:
    • Description: Water travels through the cell walls and intercellular spaces within the leaf without entering the cytoplasm.
    • Significance: Facilitates rapid water movement to areas where it is needed for transpiration.


Utilization and Loss of Water in Leaves

Photosynthesis

  • Role of Water:
    • Light-Dependent Reactions: Water is split into oxygen, protons, and electrons.
    • Products:
      • Oxygen: Released as a byproduct.
      • Protons and Electrons: Used to produce ATP and NADPH, essential for the Calvin cycle.
  • Impact:
    • Consumes water, contributing to the overall water demand within the leaf.

Transpiration

  • Definition: The process of water vapor loss from plant leaves through stomata. Reason: In order for the plant to get CO2 from the atmosphere, and to pull useful nutrients up from the soil, it has to open its guard cells.

Guard cells

  • Steps Involved:
    1. Evaporation from Mesophyll Cells:
      • Water moves from mesophyll cell walls into intercellular air spaces.
    2. Diffusion Through Stomata:
      • Water vapor diffuses out of the leaf through stomatal openings into the atmosphere.
    3. Transpirational Pull:
      • The continuous loss of water vapor creates a negative pressure (tension) that pulls more water upward through the xylem from the roots.

Mechanisms Driving Water Movement in Leaves

Cohesion and Adhesion

  • Cohesion:
    • Definition: The attraction between water molecules due to hydrogen bonding.
    • Role: Maintains a continuous water column from roots to leaves, ensuring efficient water transport.
  • Adhesion:
    • Definition: The attraction between water molecules and the hydrophilic walls of xylem vessels.
    • Role: Prevents the water column from breaking under tension, supporting the upward movement of water.

Transpirational Pull

  • Mechanism:
    • Transpiration creates a negative pressure (tension) in the leaf’s air spaces and xylem.
    • Effect: This negative pressure pulls water upward from the roots through the stem and into the leaves.

Mass Flow

  • Definition: The bulk movement of water and dissolved nutrients from the roots to the leaves through the xylem.
  • Driving Forces:
    • Cohesion-Tension Mechanism: Ensures a continuous water column.
    • Transpirational Pull: Generates the necessary force for upward movement.
  • Importance:
    • Efficiently transports large volumes of water and minerals necessary for photosynthesis and other physiological processes.

Step-by-Step Movement of Water Within Leaves

  1. Arrival at Leaf Xylem:
    • Water arrives at the leaf’s vascular bundles via xylem vessels.
  2. Exit from Xylem:
    • Water moves out of xylem vessels through pits into bundle sheath cells.
  3. Transfer to Mesophyll Cells:
    • Via symplast or apoplast pathways, water enters mesophyll cells.
  4. Utilization in Photosynthesis:
    • Water is split in the light-dependent reactions, supplying electrons and protons.
  5. Evaporation and Transpiration:
    • Excess water moves from mesophyll cells into air spaces and exits through stomata as water vapor.
  6. Generation of Transpirational Pull:
    • The loss of water vapor creates negative pressure, pulling more water into the leaf from the xylem.

Effect of Atmospheric Conditions on Water Movement

Dry Air (Low Humidity)

  • Impact:
    • Increases the water vapor gradient between the leaf and the atmosphere.
    • Enhances the rate of transpiration as water vapor diffuses more rapidly out of the leaf.
  • Result:
    • Greater transpirational pull, increasing the upward movement of water through the xylem.

Wind

  • Impact:
    • Removes the boundary layer of saturated air around the leaf surface.
    • Prevents the reabsorption of water vapor, maintaining a high transpiration rate.
  • Result:
    • Increased transpiration rate, enhancing transpirational pull and water movement.

High Temperature

  • Impact:
    • Raises the rate of evaporation from mesophyll cells.
    • Increases the kinetic energy of water molecules, facilitating faster diffusion through stomata.
  • Result:
    • Accelerated transpiration, leading to stronger transpirational pull and increased water uptake.

Low Humidity

  • Impact:
    • Similar to dry air, low humidity maintains a strong water vapor gradient.
    • Sustains high transpiration rates.
  • Result:
    • Continuous and efficient water flow from roots to leaves.

Stomatal Regulation (Guard Cells)

  • Role of Guard Cells:
    • Function: Control the opening and closing of stomata in response to environmental conditions.
  • Influence of Atmospheric Conditions:
    • Dry Conditions: Guard cells may close stomata to reduce water loss, decreasing transpiration rates.
    • Wet Conditions: Guard cells open stomata to facilitate gas exchange and transpiration.
  • Effect on Water Movement:
    • Open Stomata: Increase transpiration, enhancing transpirational pull.
    • Closed Stomata: Reduce transpiration, limiting water movement.

Negative Pressure in Water Movement

  • Definition: A pulling force generated by transpiration that creates tension within the water column in the xylem.
  • Role:
    • Drives the upward movement of water from roots to leaves against gravity.
    • Maintains the continuous flow of water through the xylem vessels.
  • Formation:
    • Occurs as water evaporates from mesophyll cells and exits through stomata, creating a suction effect.


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