7.10 Water: Leaves → atmosphere
Arrival of Water at the Leaves
- Xylem Vessels in Leaves:
- Location: Water arrives at the leaves through xylem vessels located within the vascular bundles (veins).
- Connection: Xylem vessels in leaves are continuous with those in the stem, ensuring an uninterrupted pathway for water transport.
Exit of Water from Xylem into Leaf Tissues
Movement from Xylem to Bundle Sheath Cells
- Pits in Xylem Vessel Walls:
- Description: Areas where lignin is absent, creating pores in the xylem vessel walls.
- Function: Allow water to move out of the xylem into adjacent bundle sheath cells.
- Bundle Sheath Cells:
- Role: Act as intermediary cells facilitating the transfer of water from xylem to mesophyll cells.
Pathways from Bundle Sheath to Mesophyll Cells
- Symplast Pathway:
- Description: Water moves from bundle sheath cells into mesophyll cells through plasmodesmata (cytoplasmic channels).
- Significance: Allows regulated and selective movement of water and solutes.
- Apoplast Pathway:
- Description: Water travels through the cell walls and intercellular spaces within the leaf without entering the cytoplasm.
- Significance: Facilitates rapid water movement to areas where it is needed for transpiration.
Utilization and Loss of Water in Leaves
Photosynthesis
- Role of Water:
- Light-Dependent Reactions: Water is split into oxygen, protons, and electrons.
- Products:
- Oxygen: Released as a byproduct.
- Protons and Electrons: Used to produce ATP and NADPH, essential for the Calvin cycle.
- Impact:
- Consumes water, contributing to the overall water demand within the leaf.
Transpiration
- Definition: The process of water vapor loss from plant leaves through stomata. Reason: In order for the plant to get CO2 from the atmosphere, and to pull useful nutrients up from the soil, it has to open its guard cells.
- Steps Involved:
- Evaporation from Mesophyll Cells:
- Water moves from mesophyll cell walls into intercellular air spaces.
- Diffusion Through Stomata:
- Water vapor diffuses out of the leaf through stomatal openings into the atmosphere.
- Transpirational Pull:
- The continuous loss of water vapor creates a negative pressure (tension) that pulls more water upward through the xylem from the roots.
- Evaporation from Mesophyll Cells:
Mechanisms Driving Water Movement in Leaves
Cohesion and Adhesion
- Cohesion:
- Definition: The attraction between water molecules due to hydrogen bonding.
- Role: Maintains a continuous water column from roots to leaves, ensuring efficient water transport.
- Adhesion:
- Definition: The attraction between water molecules and the hydrophilic walls of xylem vessels.
- Role: Prevents the water column from breaking under tension, supporting the upward movement of water.
Transpirational Pull
- Mechanism:
- Transpiration creates a negative pressure (tension) in the leaf’s air spaces and xylem.
- Effect: This negative pressure pulls water upward from the roots through the stem and into the leaves.
Mass Flow
- Definition: The bulk movement of water and dissolved nutrients from the roots to the leaves through the xylem.
- Driving Forces:
- Cohesion-Tension Mechanism: Ensures a continuous water column.
- Transpirational Pull: Generates the necessary force for upward movement.
- Importance:
- Efficiently transports large volumes of water and minerals necessary for photosynthesis and other physiological processes.
Step-by-Step Movement of Water Within Leaves
- Arrival at Leaf Xylem:
- Water arrives at the leaf’s vascular bundles via xylem vessels.
- Exit from Xylem:
- Water moves out of xylem vessels through pits into bundle sheath cells.
- Transfer to Mesophyll Cells:
- Via symplast or apoplast pathways, water enters mesophyll cells.
- Utilization in Photosynthesis:
- Water is split in the light-dependent reactions, supplying electrons and protons.
- Evaporation and Transpiration:
- Excess water moves from mesophyll cells into air spaces and exits through stomata as water vapor.
- Generation of Transpirational Pull:
- The loss of water vapor creates negative pressure, pulling more water into the leaf from the xylem.
Effect of Atmospheric Conditions on Water Movement
Dry Air (Low Humidity)
- Impact:
- Increases the water vapor gradient between the leaf and the atmosphere.
- Enhances the rate of transpiration as water vapor diffuses more rapidly out of the leaf.
- Result:
- Greater transpirational pull, increasing the upward movement of water through the xylem.
Wind
- Impact:
- Removes the boundary layer of saturated air around the leaf surface.
- Prevents the reabsorption of water vapor, maintaining a high transpiration rate.
- Result:
- Increased transpiration rate, enhancing transpirational pull and water movement.
High Temperature
- Impact:
- Raises the rate of evaporation from mesophyll cells.
- Increases the kinetic energy of water molecules, facilitating faster diffusion through stomata.
- Result:
- Accelerated transpiration, leading to stronger transpirational pull and increased water uptake.
Low Humidity
- Impact:
- Similar to dry air, low humidity maintains a strong water vapor gradient.
- Sustains high transpiration rates.
- Result:
- Continuous and efficient water flow from roots to leaves.
Stomatal Regulation (Guard Cells)
- Role of Guard Cells:
- Function: Control the opening and closing of stomata in response to environmental conditions.
- Influence of Atmospheric Conditions:
- Dry Conditions: Guard cells may close stomata to reduce water loss, decreasing transpiration rates.
- Wet Conditions: Guard cells open stomata to facilitate gas exchange and transpiration.
- Effect on Water Movement:
- Open Stomata: Increase transpiration, enhancing transpirational pull.
- Closed Stomata: Reduce transpiration, limiting water movement.
Negative Pressure in Water Movement
- Definition: A pulling force generated by transpiration that creates tension within the water column in the xylem.
- Role:
- Drives the upward movement of water from roots to leaves against gravity.
- Maintains the continuous flow of water through the xylem vessels.
- Formation:
- Occurs as water evaporates from mesophyll cells and exits through stomata, creating a suction effect.
Practice Questions
Question 1
Describe the structure and function of xylem vessels in leaves. (5 marks)
Mark Scheme:
- Location of Xylem Vessels:
- Xylem vessels are located within the vascular bundles (veins) of the leaves. (1 mark)
- Continuous Pathway:
- Xylem vessels in leaves are continuous with those in the stem, providing an uninterrupted pathway for water transport from roots to leaves. (1 mark)
- Cell Composition:
- Xylem vessels are composed of dead, lignified cells that form hollow tubes. (1 mark)
- Lignin in Cell Walls:
- The cell walls contain lignin, which provides strength and makes them waterproof, preventing water loss from the vessels. (1 mark)
- Facilitation of Water Transport:
- The structure of xylem vessels allows for efficient upward movement of water and dissolved minerals through the plant. (1 mark)
Question 2
Explain how water exits xylem vessels into leaf tissues. (5 marks)
Mark Scheme:
- Movement Through Pits:
- Water moves out of the xylem vessels through pits, which are unlignified areas in the xylem vessel walls. (1 mark)
- Entry into Bundle Sheath Cells:
- Through pits, water enters the bundle sheath cells, which are intermediary cells surrounding the vascular tissue. (1 mark)
- Facilitation by Bundle Sheath Cells:
- Bundle sheath cells facilitate the transfer of water from the xylem vessels into the mesophyll cells. (1 mark)
- Regulation of Water Flow:
- Bundle sheath cells regulate the movement of water and minerals, ensuring selective uptake into the leaf tissues. (1 mark)
- Connection to Mesophyll Cells:
- From bundle sheath cells, water can move into mesophyll cells via symplastic or apoplastic pathways. (1 mark)
Question 3
Compare the symplastic and apoplastic pathways in water movement within leaf tissues. (6 marks)
Mark Scheme:
- Definition of Symplastic Pathway:
- Water moves from cell to cell through the cytoplasm via plasmodesmata. (1 mark)
- Definition of Apoplastic Pathway:
- Water travels through the cell walls and intercellular spaces without entering the cytoplasm. (1 mark)
- Regulation of Water Movement:
- Symplastic Pathway allows for selective uptake and regulated movement of water and solutes. (1 mark)
- Apoplastic Pathway provides rapid movement of water but is less regulated. (1 mark)
- Role of the Endodermis:
- The Casparian strip blocks the apoplastic pathway at the endodermis, forcing water to enter the symplast. (1 mark)
- Efficiency and Speed:
- Apoplastic Pathway is faster, facilitating rapid water movement to areas needed for transpiration.
- Symplastic Pathway is slower but allows for control and selectivity. (1 mark)
Question 4
Explain the role of the endodermis and Casparian strip in regulating water and mineral uptake in dicot roots. (5 marks)
Mark Scheme:
- Location and Composition:
- The endodermis is a single layer of tightly packed cells surrounding the vascular tissues in roots.
- The Casparian strip is composed of suberin and lignin, making it waterproof. (1 mark)
- Barrier to Apoplastic Flow:
- The Casparian strip blocks the apoplastic pathway, forcing water and minerals to cross cell membranes into the symplast of endodermal cells. (1 mark)
- Selective Uptake:
- This selective barrier ensures that only necessary minerals and water are actively transported into the xylem, preventing the leakage of unwanted substances. (1 mark)
- Regulation of Ion Absorption:
- The Casparian strip allows the plant to regulate ion uptake by controlling which ions pass through the plasma membranes of endodermal cells. (1 mark)
- Prevention of Backflow:
- It prevents the backflow of solutes from the xylem into the surrounding cortex, maintaining the integrity of the water and mineral transport system. (1 mark)
Question 5
Explain how active transport of mineral ions by root hair cells affects water uptake. (5 marks)
Mark Scheme:
- Active Transport Definition:
- Active transport involves the movement of mineral ions against their concentration gradients using energy (ATP). (1 mark)
- Ion Accumulation in Root Hair Cells:
- Root hair cells actively transport ions (e.g., K⁺, Ca²⁺, NO₃⁻) into the cytoplasm, increasing the solute concentration inside the cells. (1 mark)
- Lowering of Water Potential:
- The accumulation of ions lowers the water potential (Ψ) inside the root hair cells, making it more negative compared to the surrounding soil water. (1 mark)
- Enhanced Osmotic Gradient:
- A steeper osmotic gradient is established, driving more water to enter the root hair cells via osmosis from the soil. (1 mark)
- Support for Xylem Transport:
- Increased water uptake due to active ion transport supports the continuous flow of water through the xylem, aiding overall plant hydration and nutrient transport. (1 mark)
Question 6
Describe the structure of xylem and how it is adapted for efficient water transport. (5 marks)
Mark Scheme:
- Xylem Composition:
- Xylem consists of xylem vessel elements and tracheids, which are dead, lignified cells that form continuous tubes for water transport. (1 mark)
- Cell Walls and Lignin:
- Xylem cell walls are thick and lignified, providing strength and waterproofing, preventing the collapse of vessels under negative pressure (tension). (1 mark)
- Formation of Xylem Vessels:
- Xylem vessel elements join end-to-end, with their end walls dissolving to form continuous tubes that allow uninterrupted water flow. (1 mark)
- Pits in Xylem Cells:
- Pits are unlignified areas in the xylem cell walls that allow lateral water movement between vessels and neighboring cells, maintaining water flow continuity. (1 mark)
- Narrow Diameter of Xylem Vessels:
- Xylem vessels often have a narrow diameter, which reduces the risk of air bubbles (air locks) disrupting the water column, ensuring efficient transport. (1 mark)
Question 7
Explain the cohesion-tension theory and its components in the context of water movement in plants. (6 marks)
Mark Scheme:
- Cohesion-Tension Theory Overview:
- The cohesion-tension theory explains how transpiration generates a tension (negative pressure) that pulls water upwards through the xylem. (1 mark)
- Cohesion of Water Molecules:
- Water molecules exhibit cohesion due to hydrogen bonding, creating a continuous column of water from the roots to the leaves. (1 mark)
- Adhesion to Xylem Walls:
- Adhesion of water molecules to the xylem cell walls helps stabilize the water column and prevent it from breaking under tension. (1 mark)
- Transpiration Pull:
- Transpiration at the leaf surfaces evaporates water, creating a negative pressure (tension) that pulls water upwards through the xylem. (1 mark)
- Continuous Water Flow:
- The cohesion and adhesion properties ensure a continuous flow of water, allowing for the uptake of water and dissolved minerals from the roots to the leaves. (1 mark)
- Energy-Free Process:
- The process relies on physical forces (cohesion and tension) rather than biochemical energy (ATP), making it an efficient, passive mechanism for water transport. (1 mark)
Question 8
Discuss the step-by-step process of water movement within leaves, from xylem to transpiration. (6 marks)
Mark Scheme:
- Arrival at Leaf Xylem:
- Water arrives at the leaves through xylem vessels located within the vascular bundles (veins). (1 mark)
- Exit from Xylem to Bundle Sheath Cells:
- Water moves out of the xylem vessels through pits into bundle sheath cells. (1 mark)
- Transfer to Mesophyll Cells:
- From bundle sheath cells, water moves into mesophyll cells via symplastic or apoplastic pathways. (1 mark)
- Utilization in Photosynthesis:
- Water is used in the light-dependent reactions of photosynthesis, being split into oxygen, protons, and electrons. (1 mark)
- Evaporation from Mesophyll Cells:
- Excess water moves from mesophyll cell walls into intercellular air spaces within the leaf. (1 mark)
- Diffusion Through Stomata:
- Water vapor diffuses out of the leaf through stomatal openings into the atmosphere, completing the transpiration process. (1 mark)
Question 9
Explain how environmental conditions such as temperature and wind influence the transpiration rate and water uptake in plants. (6 marks)
Mark Scheme:
- High Temperature Effects:
- Increased temperature raises the rate of water evaporation from mesophyll cells, increasing the transpiration rate. (1 mark)
- Low Humidity Effects:
- Low humidity creates a steeper water vapor gradient, enhancing the transpiration pull and increasing water uptake. (1 mark)
- Wind Effects:
- Wind removes the saturated air around the stomata, maintaining a high transpiration rate by preventing reabsorption of water vapor. (1 mark)
- Transpiration Pull Enhancement:
- Increased transpiration due to high temperature and wind strengthens the transpiration pull, promoting more efficient water transport. (1 mark)
- Water Stress Risks:
- Excessive transpiration can lead to water stress or dehydration if water uptake cannot keep pace with water loss. (1 mark)
- Adaptive Responses:
- Plants may adjust stomatal opening or alter leaf orientation in response to these factors to regulate transpiration and maintain water balance. (1 mark)
Question 10
Describe the importance of pits in xylem vessel walls for maintaining water transport in plants. (5 marks)
Mark Scheme:
- Definition of Pits:
- Pits are unlignified areas in the xylem vessel walls that create pores. (1 mark)
- Facilitation of Lateral Water Movement:
- Pits allow water to move laterally from one xylem vessel to another and into adjacent cells, maintaining continuous water flow. (1 mark)
- Prevention of Air Locks:
- By enabling lateral bypassing around obstructions, pits help prevent the formation of air bubbles (embolisms) that could disrupt the water column. (1 mark)
- Maintenance of Water Column Integrity:
- Pits support the cohesion-tension mechanism by maintaining the integrity of the water column under negative pressure. (1 mark)
- Adaptation to Tall Plants:
- In tall plants, pits ensure efficient upward transport of water over long distances, reducing the risk of transport disruptions. (1 mark)
Question 11
Describe how xylem vessels in leaves are adapted to facilitate efficient water transport. (5 marks)
Mark Scheme:
- Continuous Pathway:
- Xylem vessels in leaves are continuous with those in the stem, providing an uninterrupted pathway for water transport from roots to leaves. (1 mark)
- Lignified Cell Walls:
- Xylem vessel elements have lignified cell walls, which provide strength and waterproofing, preventing collapse under negative pressure (tension). (1 mark)
- Hollow Structure:
- Xylem vessels are hollow tubes, allowing unobstructed flow of water and dissolved minerals. (1 mark)
- Narrow Diameter:
- The narrow diameter of xylem vessels reduces the risk of air bubbles (embolisms) disrupting the water column. (1 mark)
- Pits in Vessel Walls:
- Pits are unlignified areas in the vessel walls that allow lateral water movement, maintaining continuous water flow. (1 mark)
Question 12
Explain how pits in xylem vessel walls contribute to the maintenance of the water column in plants. (5 marks)
Mark Scheme:
- Definition of Pits:
- Pits are unlignified areas in the xylem vessel walls, creating pores. (1 mark)
- Lateral Water Movement:
- Pits allow water to move laterally between adjacent xylem vessels and into neighboring cells, ensuring continuous water flow. (1 mark)
- Prevention of Air Locks:
- By enabling lateral bypassing around obstructions, pits help prevent the formation of air bubbles (embolism) that could disrupt the water column. (1 mark)
- Maintenance of Water Column Integrity:
- Pits support the cohesion-tension mechanism by maintaining the integrity of the water column under negative pressure. (1 mark)
- Adaptation to Tall Plants:
- In tall plants, pits ensure efficient upward transport of water over long distances, reducing the risk of transport disruptions. (1 mark)
Question 13
Describe the role of bundle sheath cells in the movement of water from xylem vessels to mesophyll cells. (5 marks)
Mark Scheme:
- Intermediary Role:
- Bundle sheath cells act as intermediary cells facilitating the transfer of water from xylem vessels to mesophyll cells. (1 mark)
- Water Movement Through Pits:
- Water exits the xylem vessels through pits and enters the bundle sheath cells. (1 mark)
- Symplastic and Apoplastic Pathways:
- From bundle sheath cells, water moves into mesophyll cells via symplastic or apoplastic pathways. (1 mark)
- Selective Regulation:
- Bundle sheath cells regulate the movement of water and minerals, ensuring selective uptake into the leaf tissues. (1 mark)
- Facilitation of Efficient Transport:
- They help in coordinating water flow, maintaining efficient transport to areas where it is needed for transpiration and photosynthesis. (1 mark)
Question 14
Explain how symplastic and apoplastic pathways differ in water movement within leaf tissues and their significance. (6 marks)
Mark Scheme:
- Definition of Symplastic Pathway:
- Water moves cell-to-cell through the cytoplasm via plasmodesmata. (1 mark)
- Definition of Apoplastic Pathway:
- Water travels through the cell walls and intercellular spaces without entering the cytoplasm. (1 mark)
- Regulation of Movement:
- Symplastic Pathway allows for selective uptake and regulated movement of water and solutes. (1 mark)
- Apoplastic Pathway provides rapid movement of water but is less regulated. (1 mark)
- Role of the Endodermis:
- The Casparian strip blocks the apoplastic pathway at the endodermis, forcing water to enter the symplast. (1 mark)
- Efficiency and Speed Comparison:
- Apoplastic Pathway is faster, facilitating rapid water movement to areas needed for transpiration.
- Symplastic Pathway is slower but allows for more precise control over water and nutrient uptake. (1 mark)
Question 15
Discuss how the cohesion-tension theory explains the upward movement of water in plants. (6 marks)
Mark Scheme:
- Cohesion-Tension Theory Overview:
- The cohesion-tension theory explains how transpiration generates a tension (negative pressure) that pulls water upwards through the xylem. (1 mark)
- Cohesion of Water Molecules:
- Water molecules exhibit cohesion due to hydrogen bonding, creating a continuous column of water from the roots to the leaves. (1 mark)
- Adhesion to Xylem Walls:
- Adhesion of water molecules to the xylem cell walls helps stabilize the water column and prevent it from breaking under tension. (1 mark)
- Transpiration Pull:
- Transpiration at the leaf surfaces evaporates water, creating a negative pressure (tension) that pulls water upwards through the xylem. (1 mark)
- Continuous Water Flow:
- The cohesion and adhesion properties ensure a continuous flow of water, allowing for the uptake of water and dissolved minerals from the roots to the leaves. (1 mark)
- Energy-Free Process:
- The process relies on physical forces (cohesion and tension) rather than biochemical energy (ATP), making it an efficient, passive mechanism for water transport. (1 mark)
Question 16
Explain how transpiration creates a negative pressure in the xylem and its effect on water movement. (5 marks)
Mark Scheme:
- Definition of Transpiration:
- Transpiration is the loss of water vapor from stomata in leaves. (1 mark)
- Creation of Negative Pressure:
- As water vapor exits the leaf, it evaporates from the mesophyll cells into the air spaces, creating a negative pressure (tension) in the xylem. (1 mark)
- Transpiration Pull:
- This negative pressure pulls water upwards through the xylem vessels from the roots to the leaves. (1 mark)
- Cohesion-Tension Mechanism:
- The cohesion between water molecules maintains the continuous water column, ensuring that the pull affects the entire column from roots to leaves. (1 mark)
- Effect on Water Movement:
- The negative pressure generated by transpiration drives the mass flow of water and dissolved minerals against gravity, maintaining the plant’s hydration and nutrient supply. (1 mark)
Question 17
Describe how guard cells regulate transpiration and the impact on water movement within the plant. (5 marks)
Mark Scheme:
- Function of Guard Cells:
- Guard cells control the opening and closing of stomata. (1 mark)
- Stomatal Opening:
- When guard cells take up potassium ions (K⁺) and water, they inflate, causing the stomata to open. (1 mark)
- Stomatal Closing:
- Under water stress or high salt concentrations, guard cells lose K⁺ ions and water, causing them to deflate and close the stomata. (1 mark)
- Regulation of Transpiration Rate:
- Open stomata increase transpiration rates, enhancing the transpiration pull and water uptake.
- Closed stomata reduce transpiration, conserving water within the plant. (1 mark)
- Impact on Water Movement:
- By regulating stomatal aperture, guard cells balance the need for CO₂ uptake for photosynthesis with the need to conserve water, thus controlling water movement within the plant. (1 mark)
Question 18
Explain how environmental factors such as temperature and wind influence the transpiration rate and consequently water uptake in plants. (6 marks)
Mark Scheme:
- High Temperature Effects:
- Increased temperature raises the rate of water evaporation from mesophyll cells, increasing the transpiration rate. (1 mark)
- Low Humidity Effects:
- Low humidity creates a steeper water vapor gradient, enhancing the transpiration pull and increasing water uptake. (1 mark)
- Wind Effects:
- Wind removes the saturated air around the stomata, maintaining a high transpiration rate by preventing reabsorption of water vapor. (1 mark)
- Transpiration Pull Enhancement:
- Increased transpiration due to high temperature and wind strengthens the transpiration pull, promoting more efficient water transport. (1 mark)
- Water Stress Risks:
- Excessive transpiration can lead to water stress or dehydration if water uptake cannot keep pace with water loss. (1 mark)
- Adaptive Responses:
- Plants may adjust stomatal opening or alter leaf orientation in response to these factors to regulate transpiration and maintain water balance. (1 mark)
Question 19
Describe the step-by-step process of water movement within leaves, from xylem to transpiration. (6 marks)
Mark Scheme:
- Arrival at Leaf Xylem:
- Water arrives at the leaves through xylem vessels located within the vascular bundles (veins). (1 mark)
- Exit from Xylem to Bundle Sheath Cells:
- Water moves out of the xylem vessels through pits into bundle sheath cells. (1 mark)
- Transfer to Mesophyll Cells:
- From bundle sheath cells, water moves into mesophyll cells via symplastic or apoplastic pathways. (1 mark)
- Utilization in Photosynthesis:
- Water is used in the light-dependent reactions of photosynthesis, being split into oxygen, protons, and electrons. (1 mark)
- Evaporation from Mesophyll Cells:
- Excess water moves from mesophyll cell walls into intercellular air spaces within the leaf. (1 mark)
- Diffusion Through Stomata:
- Water vapor diffuses out of the leaf through stomatal openings into the atmosphere, completing the transpiration process. (1 mark)
Question 20
Explain how the structure of bundle sheath cells facilitates the transfer of water from xylem vessels to mesophyll cells. (5 marks)
Mark Scheme:
- Location within Vascular Bundles:
- Bundle sheath cells are located surrounding the vascular tissues (xylem and phloem) within the leaf’s vascular bundles. (1 mark)
- Intermediary Role:
- They act as intermediary cells, facilitating the transfer of water from xylem vessels to mesophyll cells. (1 mark)
- Plasmodesmata Presence:
- Bundle sheath cells contain plasmodesmata, allowing symplastic movement of water into mesophyll cells. (1 mark)
- Selective Regulation:
- They regulate the movement of water and minerals, ensuring that only necessary substances are transferred to the mesophyll. (1 mark)
- Support of Photosynthetic Processes:
- By controlling water flow, bundle sheath cells maintain optimal hydration of mesophyll cells, supporting efficient photosynthesis. (1 mark)
Quizzes
Quiz 1
Quiz 2